ePPEplus-enRERV(for plant)

LOCUS       ePPEplus-enRERV(for plant)       11700 bp DNA     circular SYN 27-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ePPEplus-enRERV(for plant)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   LQP
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2026 from SnapGene 7.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1999
                     /label=ZmUbi1
     CDS             2021..2047
                     /codon_start=1
                     /product="nuclear localization signal of human c-myc (Dang 
                     and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2087..2107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2108..6208
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=nCas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6215..6262
                     /codon_start=1
                     /label=XTEN
                     /translation="SGSETPGTSESATPES"
     CDS             6263..6430
                     /codon_start=1
                     /label=NC
                     /translation="ATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDCPKKPRG
                     PRGPRPQTSLL"
     CDS             6431..6451
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     CDS             6458..6553
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6557..8617
                     /codon_start=1
                     /label=enRERV
                     /translation="PPVACLVLSLEEEYRLHEQNPKQLLAPEWLSAFSEVWAEQTGMGL
                     AKQVPPVVVELKADASPVSVKQRPMSREAKEGIRPHIQRLLQLGILVPCQSPWNTPLLP
                     VRKPGTNDYRPVQDLREVNKRVQDIHPTVPNPYNLLSSLPPERTFYTVLDLKDAFFCLR
                     LHPNSQPLFAFEWRDPEGGHTGQLTWTRLPQGFKNSPTLFNEALHRDLAPFRAQNPQIS
                     LLQYADDLLLAASTQELCREGTKRLLNELGELGYRVSAKKAQLCRTEVTYLGYTLREGK
                     RWLTEARKKTVMQIPTPTTPRQVREFLGKAGFCRLFIPGFATLAAPLYLLTKPKVPFTW
                     TEEHQRAFEDIKAALLAAPALALPDLTKPFTLYVDERAGVARGVLTQALGPWKRPVAYL
                     SKKLDPVASGWPSRLKAIAAVALLVKDADKLTLGQHVTVIAPHALESIVRQPPDRWMTN
                     ARMTHYQSLLLNERVTFAPPAILNPATLLPEIHNSTPIHQCVDILAEETGTRKDLTDRP
                     WPGVPAWYTDGSSFVVEGKRRAGAAVVDGKQVIWASSLPEGTSAQKAELVALTQALRLA
                     EGKAINIYTDSRYAFATAHIHGAIYKQRGLLTSAGRDIKNKEEILALLEAVHLPKKVAI
                     IHCPGHQKGEDPITKGNQMADLVAKQVAQQVTILAEKSQTPVKTPVTDDSNY"
     CDS             8717..8737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8800..8976
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9530..9546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9554..9570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9578..9608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9623..9644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9932..10520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10691..11551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11552..11656)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttactgg ttgtacaatg ccggcggcca agagagtcaa
     2041 gctcgacggc gggaagcgca cagctgatgg ttctgaattc gagtccccta agaaaaagag
     2101 aaaagtggac aagaagtact cgatcggcct cgatattggg actaactctg ttggctgggc
     2161 cgtgatcacc gacgagtaca aggtgccctc aaagaagttc aaggtcctgg gcaacaccga
     2221 tcggcattcc atcaagaaga atctcattgg cgctctcctg ttcgacagcg gcgagacggc
     2281 tgaggctacg cggctcaagc gcaccgcccg caggcggtac acgcgcagga agaatcgcat
     2341 ctgctacctg caggagattt tctccaacga gatggcgaag gttgacgatt ctttcttcca
     2401 caggctggag gagtcattcc tcgtggagga ggataagaag cacgagcggc atccaatctt
     2461 cggcaacatt gtcgacgagg ttgcctacca cgagaagtac cctacgatct accatctgcg
     2521 gaagaagctc gtggactcca cagataaggc ggacctccgc ctgatctacc tcgctctggc
     2581 ccacatgatt aagttcaggg gccatttcct gatcgagggg gatctcaacc cggacaatag
     2641 cgatgttgac aagctgttca tccagctcgt gcagacgtac aaccagctct tcgaggagaa
     2701 ccccattaat gcgtcaggcg tcgacgcgaa ggctatcctg tccgctaggc tctcgaagtc
     2761 tcggaagctc gagaacctga tcgcccagct gccgggcgag aagaagaacg gcctgttcgg
     2821 gaatctcatt gcgctcagcc tggggctcac gcccaacttc aagtcgaatt tcgatctcgc
     2881 tgaggacgcc aagctgcagc tctccaagga cacatacgac gatgacctgg ataacctcct
     2941 ggcccagatc ggcgatcagt acgcggacct gttcctcgct gccaagaatc tgtcggacgc
     3001 catcctcctg tctgatattc tcagggtgaa caccgagatt acgaaggctc cgctctcagc
     3061 ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga aggcgctggt
     3121 caggcagcag ctccccgaga agtacaagga gatcttcttc gatcagtcga agaacggcta
     3181 cgctgggtac attgacggcg gggcctctca ggaggagttc tacaagttca tcaagccgat
     3241 tctggagaag atggacggca cggaggagct gctggtgaag ctcaagcgcg aggacctcct
     3301 gaggaagcag cggacattcg ataacggcag catcccacac cagattcatc tcggggagct
     3361 gcacgctatc ctgaggaggc aggaggactt ctaccctttc ctcaaggata accgcgagaa
     3421 gatcgagaag attctgactt tcaggatccc gtactacgtc ggcccactcg ctaggggcaa
     3481 ctcccgcttc gcttggatga cccgcaagtc agaggagacg atcacgccgt ggaacttcga
     3541 ggaggtggtc gacaagggcg ctagcgctca gtcgttcatc gagaggatga cgaatttcga
     3601 caagaacctg ccaaatgaga aggtgctccc taagcactcg ctcctgtacg agtacttcac
     3661 agtctacaac gagctgacta aggtgaagta tgtgaccgag ggcatgagga agccggcttt
     3721 cctgtctggg gagcagaaga aggccatcgt ggacctcctg ttcaagacca accggaaggt
     3781 cacggttaag cagctcaagg aggactactt caagaagatt gagtgcttcg attcggtcga
     3841 gatctctggc gttgaggacc gcttcaacgc ctccctgggg acctaccacg atctcctgaa
     3901 gatcattaag gataaggact tcctggacaa cgaggagaat gaggatatcc tcgaggacat
     3961 tgtgctgaca ctcactctgt tcgaggaccg ggagatgatc gaggagcgcc tgaagactta
     4021 cgcccatctc ttcgatgaca aggtcatgaa gcagctcaag aggaggaggt acaccggctg
     4081 ggggaggctg agcaggaagc tcatcaacgg cattcgggac aagcagtccg ggaagacgat
     4141 cctcgacttc ctgaagagcg atggcttcgc gaaccgcaat ttcatgcagc tgattcacga
     4201 tgacagcctc acattcaagg aggatatcca gaaggctcag gtgagcggcc agggggactc
     4261 gctgcacgag catatcgcga acctcgctgg ctcgccagct atcaagaagg ggattctgca
     4321 gaccgtgaag gttgtggacg agctggtgaa ggtcatgggc aggcacaagc ctgagaacat
     4381 cgtcattgag atggcccggg agaatcagac cacgcagaag ggccagaaga actcacgcga
     4441 gaggatgaag aggatcgagg agggcattaa ggagctgggg tcccagatcc tcaaggagca
     4501 cccggtggag aacacgcagc tgcagaatga gaagctctac ctgtactacc tccagaatgg
     4561 ccgcgatatg tatgtggacc aggagctgga tattaacagg ctcagcgatt acgacgtcga
     4621 tgccatcgtt ccacagtcat tcctgaagga tgactccatt gacaacaagg tcctcaccag
     4681 gtcggacaag aaccggggca agtctgataa tgttccttca gaggaggtcg ttaagaagat
     4741 gaagaactac tggcgccagc tcctgaatgc caagctgatc acgcagcgga agttcgataa
     4801 cctcacaaag gctgagaggg gcgggctctc tgagctggac aaggcgggct tcatcaagag
     4861 gcagctggtc gagacacggc agatcactaa gcacgttgcg cagattctcg actcacggat
     4921 gaacactaag tacgatgaga atgacaagct gatccgcgag gtgaaggtca tcaccctgaa
     4981 gtcaaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc gggagatcaa
     5041 caattaccac catgcccatg acgcgtacct gaacgcggtg gtcggcacag ctctgatcaa
     5101 gaagtaccca aagctcgaga gcgagttcgt gtacggggac tacaaggttt acgatgtgag
     5161 gaagatgatc gccaagtcgg agcaggagat tggcaaggct accgccaagt acttcttcta
     5221 ctctaacatt atgaatttct tcaagacaga gatcactctg gccaatggcg agatccggaa
     5281 gcgccccctc atcgagacga acggcgagac gggggagatc gtgtgggaca agggcaggga
     5341 tttcgcgacc gtcaggaagg ttctctccat gccacaagtg aatatcgtca agaagacaga
     5401 ggtccagact ggcgggttct ctaaggagtc aattctgcct aagcggaaca gcgacaagct
     5461 catcgcccgc aagaaggact gggatccgaa gaagtacggc gggttcgaca gccccactgt
     5521 ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga agctcaagag
     5581 cgtgaaggag ctgctgggga tcacgattat ggagcgctcc agcttcgaga agaacccgat
     5641 cgatttcctg gaggcgaagg gctacaagga ggtgaagaag gacctgatca ttaagctccc
     5701 caagtactca ctcttcgagc tggagaacgg caggaagcgg atgctggctt ccgctggcga
     5761 gctgcagaag gggaacgagc tggctctgcc gtccaagtat gtgaacttcc tctacctggc
     5821 ctcccactac gagaagctca agggcagccc cgaggacaac gagcagaagc agctgttcgt
     5881 cgagcagcac aagcattacc tcgacgagat cattgagcag atttccgagt tctccaagcg
     5941 cgtgatcctg gccgacgcga atctggataa ggtcctctcc gcgtacaaca agcaccgcga
     6001 caagccaatc agggagcagg ctgagaatat cattcatctc ttcaccctga cgaacctcgg
     6061 cgcccctgct gctttcaagt acttcgacac aactatcgat cgcaagaggt acacaagcac
     6121 taaggaggtc ctggacgcga ccctcatcca ccagtcgatt accggcctct acgagacgcg
     6181 catcgacctg tctcagctcg ggggcgacga attctccggg agcgagacgc caggcacctc
     6241 cgagtcggcc accccagaat ctgccacagt ggtgtccggc caaaagcagg accgccaggg
     6301 cggagaacgc agaaggtccc agctcgatag ggatcagtgt gcctactgca aggagaaggg
     6361 ccactgggcc aaagactgcc cgaaaaagcc gcgcggccca cgcggcccaa ggccacaaac
     6421 atccctcctt ccaaagaaga agcggaaggt ggagctcagc ggaggatctt ccggaggatc
     6481 tagcggctcc gagacaccag gaacatccga aagcgctaca ccagaatcta gcggaggctc
     6541 ttccggagga tctaggcctc ccgtcgcgtg cctcgtactg agcctagagg aggagtacag
     6601 gctgcatgag caaaatccga agcagctgct agcccctgaa tggctgagcg ccttctccga
     6661 ggtatgggcg gagcagactg gcatgggact ggccaagcag gtgcctcctg tggtggtaga
     6721 gctgaaggcg gacgccagcc cagtgagcgt gaaacaacgc ccgatgtcga gggaagcaaa
     6781 ggaaggcatt agaccccaca tccagagact gctgcaattg ggaatcctgg taccctgcca
     6841 aagcccttgg aacacgccac tgctgcctgt gcgcaaacca ggaactaatg attacagacc
     6901 tgtacaggat ctaagggaag ttaacaagcg ggtgcaggac atacatccta cggtcccaaa
     6961 cccctacaac ttgctgagct ctctgccccc cgagcgcaca ttctacactg tgctcgactt
     7021 aaaagatgcg ttcttctgcc ttcgcctcca cccgaactca cagccactgt tcgcgttcga
     7081 gtggcgcgac cctgagggcg gccacaccgg gcaactgacg tggacacggc tgccacaggg
     7141 tttcaagaac agccctactc tatttaacga ggccctgcac cgtgatctgg cccccttccg
     7201 ggcgcagaac cctcaaatca gtctactgca gtatgcggat gacctgctgc tagccgcttc
     7261 tactcaggag ctctgccggg agggcacaaa gcggctgtta aatgagctgg gcgagctggg
     7321 ataccgcgtg tctgccaaga aggcacaatt gtgtcggaca gaggtgacat atctcggcta
     7381 cacattaaga gaaggtaaga gatggctcac tgaagcacgt aagaagactg tcatgcagat
     7441 tcctacccct accacgcccc gtcaggtgcg ggagttcctc ggcaaggccg gcttctgtag
     7501 actgtttatt cctggatttg ccaccctggc cgccccactc tacctgctga cgaagccaaa
     7561 ggttcctttc acctggactg aggaacatca gcgggctttc gaagacatca aagccgccct
     7621 gctggcggct ccagctctgg ccctccctga cctcaccaag cccttcacac tctatgtgga
     7681 cgagagagct ggagtggcca gaggtgtgct gacacaggca ctggggccgt ggaagagacc
     7741 cgtggcgtac ctatctaaaa agctcgaccc cgtggcctct gggtggccga gccgcctaaa
     7801 ggccatcgct gctgtagctc tactggtcaa agatgctgac aagctgaccc tgggccagca
     7861 tgtcaccgtc atcgccccgc acgccttaga atccatagtc cggcaaccac cagatcggtg
     7921 gatgacaaac gctaggatga cgcattatca gagcttgctg ttaaacgaaa gagtgacatt
     7981 tgcgccccct gccatcctta acccagcgac gctactgccg gagatccaca actcaacccc
     8041 catccaccag tgcgtcgaca ttctggctga ggagaccggc acgcgcaagg acctcacgga
     8101 cagaccatgg cctggtgtgc cggcatggta caccgacggc agctcctttg tcgtggaggg
     8161 aaagcgacgc gcaggcgcgg ctgtggtgga cggcaagcaa gtcatctggg catcctccct
     8221 gccagaaggc accagcgctc agaaagcgga gttggtggcc ttgacccagg ccctcagatt
     8281 agctgaaggg aaggctatta atatctacac cgattctaga tacgccttcg ccacggcgca
     8341 catccacggc gcgatctaca agcagcgggg cctgctgacc agtgccgggc gagacatcaa
     8401 gaataaggaa gagattcttg ccctgctgga ggccgtccac ctccccaaaa aggtggccat
     8461 tatccactgc cccggccacc agaagggaga ggatccaatt acaaagggga accagatggc
     8521 cgacctcgtg gcgaagcagg tcgctcagca ggttaccatc ctggcagaaa aatcgcagac
     8581 ccctgtgaag acacctgtga cagatgattc taactatagc ggcggcagca aaagaacggc
     8641 ggacggctct gagaagcgca ccgctgatag ccagcattca actcctccga aaacaaagag
     8701 gaaagttgag ttcgaaccga agaagaaaag gaaggtgtga tgagcggccg cggtacgctg
     8761 aaatcaccag tctctctcta caaatctatc tctctctatt ttctccataa ataatgtgtg
     8821 agtagtttcc cgataaggga aattagggtt cttatagggt ttcgctcatg tgttgagcat
     8881 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     8941 ttcctaaaac caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg
     9001 tcgtgaatat aaaccagaca cgagacgact aaacctggag cccagacgcc gttcgaagct
     9061 agaagtaccg cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg
     9121 ttgactcccc cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac
     9181 aaggaaggat aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta
     9241 cgctactata cttatactat acgctaaggg aatgcttgta tttataccct atacccccta
     9301 ataacccctt atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca
     9361 gagccatgaa taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa
     9421 gagttcttaa ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg
     9481 tgacggggat cgcatgcgat atctcgagat ctagcttggc gtaatcatgg tcatagctgt
     9541 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9601 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9661 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9721 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9781 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9841 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9901 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9961 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10021 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10081 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10141 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10201 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10261 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10321 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10381 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10441 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10501 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10561 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10621 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10681 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10741 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10801 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10861 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10921 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10981 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11041 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11101 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11161 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11221 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11281 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11341 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11401 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11461 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11521 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11641 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgccagtgcc aagctaattc
//